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Current Programs of the Post-ISCF Symposium
New
Frontiers in Human Stem Cell Research
April 19-23, 2008
Institute of Zoology, Chinese Academy of Sciences, Beijing
Part I: Regulatory, Ethical and Social Issues
April 19, 2008, Saturday
I Stem Cell Regulatory Bodies
Stem Cell Policy - A Global perspective
WHO
Stem Cell Policy - A US perspective
FDA
Stem Cell Policy - A UK and European perspective
MHRA/EMEA
Stem Cell Policy - A China or Asia perspective
MOH/CSSCR (Ministry of Health and Chinese Society for Stem Cell Research)
Fundamental Issues for GMP quality
management systems
P Carter
II International Collaboration (Society or organizational aspects)
ISCF projects
G. Stacey. In liaison with P Andrews
hescreg project: a database of international stem cell lines
G Stacey
ISSCR - International Affairs Committee
H Rooke
SNAP
D Sipp
III Stem Cell Ethics and delivery of products
ISCF-EWP
R Isasi on behalf of B Knoppers
Life, Science and Ethics: Frontier Issues in the Ethics of Stem Cell Research
I Hyun (Case Western Reserve University)
Clinical Grade Stem Cells Banking: ESI a Case study
J Crook
IV Social and Other Issues
International Patent Landscape
C Morris
Press and how the media may change the way the society view Stem Cell research
Patient Aspects
B Siegel (Genetics Policy Institute)
Funding Opportunities: CIRM, UKSCN and
Toucan
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Part II: From Fundamental Biology to Clinical Application
April 20, 2008, Sunday
I Stem Cell Culture: Beyond the Basics
8:30-9:15am Good Cell Culture Practice in Mammalian Cell Cultures
9:15-10:00 am Validation and Qualification
of GMP Facilities
II Basic Stem Cell Biology: Identification and Confirmation
10:15-11:15am Important Issues and QC in the Growth and Maintenance of hES Cells
11:15-11:45am Cell Surface Markers on Human Stem Cells
III Stem Cell Biology: Pluripotency
1:00-1:30pm Teratomas in vivo differentiation
1:30-2:00pm Neuronal Differentiation and Application
2:00-2:30pm Hematopoitic stem cell differentiation and application
2:30-3:00pm Differentiation into Mesenchymal and other lineage
IV New Advances in Stem Cell Technology and Regeneration Medicine
3:15-3:50pm Nuclear Transfer and Therapeutic Cloning
3:50-4:30pm iPS
4:30-5:10pm Disease Models for Stem Cell Research
5:10-5:50pm Epigenetic regulations
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Part III: Practical Aspects - A Training Course in hESC Culture and Application
April 21 - 23, 2008, Monday - Wednesday
Day 1 (April 21):
8:30am - 9:00am General considerations:
Laboratory safety and guidelines
Reagent preparation
Concerns regarding hESC for fundamental studies vs. clinical applications
9:00am - 10:30am Demonstrate human feeder harvesting and practice MEF harvesting
10:30am - 12:00pm Thawing MEFs, (inactivation) and plating feeders
Lunch break
1:00pm - 5:30pm Plating hESC, and demonstrations of Immunofluorescence, Flowcytometry
(Karyotyping)
Day 2 (April 22):
8:30am - 10:30am Passaging hESC (Enzymatic and Mechanical)
10:30am - 12:00pm hESC morphologies, demonstration
Lunch break
1:00pm - 5:30pm Picking differentiated ES cells, culturing ES cells under defined conditions
(embryo bodies)
Freezing hESC and MEFs
Day 3 (April 23):
8:30am - 10:30am Feeder-free hESC culturing
10:30am - 12:00pm Overview of iPS strategies
Lunch break
1:00pm - 5:30pm Differentiation Techniques and creating cell banks
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